Source Paper
Lack of striatal-enriched protein tyrosine phosphatase affected the serotonin system, behavior, and brain morphology in mice
Moskaliuk V, Komleva P, Khotskin N, Arefieva A, Shevelev O et al.
Front Psychiatry • 2026
Open Field Test
Objective: To assess the locomotor and exploratory activity of mice
This is a Open Field Test protocol using mice as the model organism. The procedure involves 4 procedural steps, 3 equipment items, 2 materials. Extracted from a 2026 paper published in Front Psychiatry.
Model and subjects
mice • C57BL/6-Ptpn5_KO and C57BL/6 • male • 2-month-old adult • not specified
Study window
~5 minutes hands-on
Core workflow
Place mouse in arena • Record movement • Manual behavioral scoring
Primary readouts
- Total distance traveled (m)
- Time (%) spent in the center of the arena
- Number of vertical postures (rearing)
- Number of grooming episodes
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Place mouse in arena
Place the mouse in the circular arena (60 cm diameter)
View evidence from paper
“The test took place at the circular arena, 60 cm in diameter”
Record movement
Automatically trace mouse movement for 5 minutes using a digital camera
View evidence from paper
“Movement of the mouse was automatically traced for 5 min with a digital camera”
Manual behavioral scoring
An experienced rater blind to experimental groups counts vertical postures (rearing) and grooming episodes
View evidence from paper
“The number of vertical postures (rearing) and the number of grooming episodes were marked by experienced rater blinded to the experimental group assignment”
Clean arena
Clean the arena with wet H2O2 and dry napkins after each test
View evidence from paper
“After each test, the arena was cleaned with wet (H 2 O 2 ) and dry napkins”