Source Paper
Lack of striatal-enriched protein tyrosine phosphatase affected the serotonin system, behavior, and brain morphology in mice
Moskaliuk V, Komleva P, Khotskin N, Arefieva A, Shevelev O et al.
Front Psychiatry • 2026
Open Field Test
behavioralmiceC57BL/6-Ptpn5_KO and C57BL/6
Objective: To assess the locomotor and exploratory activity of mice
Materials & Equipment Checklist
5 items
Protocol Steps
1
Place mouse in arena
Place the mouse in the circular arena (60 cm diameter)
View evidence from paper
“The test took place at the circular arena, 60 cm in diameter”
2
Record movement
Automatically trace mouse movement for 5 minutes using a digital camera
View evidence from paper
“Movement of the mouse was automatically traced for 5 min with a digital camera”
3
Manual behavioral scoring
An experienced rater blind to experimental groups counts vertical postures (rearing) and grooming episodes
View evidence from paper
“The number of vertical postures (rearing) and the number of grooming episodes were marked by experienced rater blinded to the experimental group assignment”
4
Clean arena
Clean the arena with wet H2O2 and dry napkins after each test
View evidence from paper
“After each test, the arena was cleaned with wet (H 2 O 2 ) and dry napkins”