Source Paper
Systemic Interplay of BDNF and Serotonin Pathways Defines Behavioral and Molecular Responses to Midbrain 5-HT7 Overexpression and Chronic Ethanol Consumption
Rodnyy A, Oreshko A, Eremin D, Naumenko V, Bazovkina D
Biomolecules • 2026
Open Field Test
behavioralmiceC57BL/6
Objective: To assess horizontal locomotor activity in mice using an open field test
Protocol Steps
1
Setup arena lighting
Position two 12 W halogen lamps 40 cm beneath the arena floor to illuminate the semi-transparent floor
View evidence from paper
“two 12 W halogen lamps positioned 40 cm beneath the arena floor”
2
Place mouse in arena
Place each mouse near the wall of the circular arena
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“Each mouse was placed near the wall”
3
Record behavior
Record mouse behavior for 5 minutes using a digital video camera positioned 80 cm from the arena
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“its behavior was recorded for 5 min using a digital video camera (Sony, Japan) located 80 cm from the arena”
4
Clean arena
Clean the arena after each test session
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“The arena was cleaned after each test session”
5
Analyze video footage
Analyze video footage frame by frame using EthoStudio software 2.0 to automatically measure horizontal locomotor activity
View evidence from paper
“Video footage was analyzed frame by frame using custom EthoStudio software 2.0. Horizontal locomotor activity (path length) was automatically measured”