Source Paper
Systemic Interplay of BDNF and Serotonin Pathways Defines Behavioral and Molecular Responses to Midbrain 5-HT7 Overexpression and Chronic Ethanol Consumption
Rodnyy A, Oreshko A, Eremin D, Naumenko V, Bazovkina D
Biomolecules • 2026
Open Field Test
Objective: To assess horizontal locomotor activity in mice using an open field test
This is a Open Field Test protocol using mice as the model organism. The procedure involves 5 procedural steps, 2 equipment items, 3 materials. Extracted from a 2026 paper published in Biomolecules.
Model and subjects
mice • C57BL/6 • male • 8-10 weeks • 65
Study window
~5 minutes hands-on
Core workflow
Setup arena lighting • Place mouse in arena • Record behavior
Primary readouts
- Horizontal locomotor activity (path length)
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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Protocol Steps
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Setup arena lighting
Position two 12 W halogen lamps 40 cm beneath the arena floor to illuminate the semi-transparent floor
View evidence from paper
“two 12 W halogen lamps positioned 40 cm beneath the arena floor”
Place mouse in arena
Place each mouse near the wall of the circular arena
View evidence from paper
“Each mouse was placed near the wall”
Record behavior
Record mouse behavior for 5 minutes using a digital video camera positioned 80 cm from the arena
View evidence from paper
“its behavior was recorded for 5 min using a digital video camera (Sony, Japan) located 80 cm from the arena”
Clean arena
Clean the arena after each test session
View evidence from paper
“The arena was cleaned after each test session”
Analyze video footage
Analyze video footage frame by frame using EthoStudio software 2.0 to automatically measure horizontal locomotor activity
View evidence from paper
“Video footage was analyzed frame by frame using custom EthoStudio software 2.0. Horizontal locomotor activity (path length) was automatically measured”