Source Paper
Ablation of Unilateral Hippocampal GABAergic Neurons: A Novel Mouse Model of Mesial Temporal Lobe Epilepsy With Hippocampal Sclerosis
Tang T, Xu J, Fu B, Geng C, Li Y et al.
CNS Neurosci Ther • 2026
Open-Field Test
Objective: To evaluate anxiety-like behaviors and locomotor activity in epilepsy animal models during inter-ictal periods
This is a Open-Field Test protocol using mice as the model organism. The procedure involves 3 procedural steps, 1 equipment items, 1 materials. Extracted from a 2026 paper published in CNS Neurosci Ther.
Model and subjects
mice • C57BL/6 and VGAT‐ires‐Cre • Male • approximately 8 weeks old • not specified
Study window
~40 minutes hands-on
Core workflow
Habituation • Testing phase • Data collection
Primary readouts
- Total distance traveled (locomotor activity)
- Time spent in the central zone (anxiety-like behavior)
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Habituation
Acclimatize mice to the test arena for 30 minutes prior to testing
View evidence from paper
“mice are acclimatized to the test arena for 30 min prior to the test for habituation”
Testing phase
Place a single mouse in the center of the arena and allow free exploration for 10 minutes
View evidence from paper
“a single mouse is placed in the center of the arena and allowed to explore freely for 10 min”
Data collection
Collect locomotor activity (total distance traveled) and anxiety-like behavior (time spent in central zone) data
View evidence from paper
“Data including locomotor activity (total distance traveled) and anxiety‐like behavior (time spent in the central zone) were collected”