Source Paper
Ablation of Unilateral Hippocampal GABAergic Neurons: A Novel Mouse Model of Mesial Temporal Lobe Epilepsy With Hippocampal Sclerosis
Tang T, Xu J, Fu B, Geng C, Li Y et al.
CNS Neurosci Ther • 2026
Open-Field Test
behavioralmiceC57BL/6 and VGAT‐ires‐Cre
Objective: To evaluate anxiety-like behaviors and locomotor activity in epilepsy animal models during inter-ictal periods
Materials & Equipment Checklist
2 items
Gather these items before starting the experiment. Check off items as you prepare.
Equipment1
not specified
Materials1
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Protocol Steps
1
Habituation
Acclimatize mice to the test arena for 30 minutes prior to testing
View evidence from paper
“mice are acclimatized to the test arena for 30 min prior to the test for habituation”
2
Testing phase
Place a single mouse in the center of the arena and allow free exploration for 10 minutes
View evidence from paper
“a single mouse is placed in the center of the arena and allowed to explore freely for 10 min”
3
Data collection
Collect locomotor activity (total distance traveled) and anxiety-like behavior (time spent in central zone) data
View evidence from paper
“Data including locomotor activity (total distance traveled) and anxiety‐like behavior (time spent in the central zone) were collected”