Source Paper
Lack of striatal-enriched protein tyrosine phosphatase affected the serotonin system, behavior, and brain morphology in mice
Moskaliuk V, Komleva P, Khotskin N, Arefieva A, Shevelev O et al.
Front Psychiatry • 2026
Rotarod Test
Objective: To assess balance and motor coordination
This is a Rotarod Test protocol using mice as the model organism. The procedure involves 4 procedural steps, 1 equipment items. Extracted from a 2026 paper published in Front Psychiatry.
Model and subjects
mice • C57BL/6-Ptpn5_KO and C57BL/6 • male • 2-month-old • not specified
Study window
~5 minutes hands-on
Core workflow
Place mouse on rotating rod • Record fall parameters • Repeat test with intervals
Primary readouts
- latency time (s)
- rotation frequency (rpm) at falling
- mean fall latency of three trials
Key equipment and reagents
Verified items
0
Direct vendor links
0
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Protocol Steps
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Place mouse on rotating rod
A mouse was placed on the rod with rotation frequency gradually increasing from 5 to 40 rpm within 5 min
View evidence from paper
“A mouse was placed on the rod with a rotation frequency gradually increasing from 5 to 40 rpm within 5 min”
Record fall parameters
The latency time (s) and rotation frequency (rpm) of the mouse's falling were registered automatically by the device software
View evidence from paper
“The latency time (s) and rotation frequency (rpm) of the mouse's falling were registered automatically by the device software”
Repeat test with intervals
For each mouse, the test was repeated three times with 1-min interval
View evidence from paper
“For each mouse, the test was repeated three times with 1-min interval”
Calculate mean fall latency
The mean fall latency of the three trials was taken as the final parameter
View evidence from paper
“The mean fall latency of the three trials was taken as the final parameter”