Source Paper
Behavioral effects of a chronic envy-like stress paradigm in mice using an adjacent cage model
Ueno H, Tanaka Y, Kitano E, Takahashi Y, Mori S et al.
IBRO Neurosci Rep • 2026
Social interaction test in a novel environment
Objective: Assess social behavior in a novel environment
This is a Social interaction test in a novel environment protocol using mice as the model organism. The procedure involves 4 procedural steps, 2 equipment items, 2 materials. Extracted from a 2026 paper published in IBRO Neurosci Rep.
Model and subjects
mice • C57BL/6 N • male • Eight-week-old • 20
Study window
~10 minutes hands-on
Core workflow
Setup testing environment • Place mice in arena • Allow free interaction
Primary readouts
- total distance traveled (m)
- number of contacts
- total contact time (s)
- mean duration per contact (s)
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
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Setup testing environment
Set up square white acrylic box with proper illumination
View evidence from paper
“apparatus consisted of a square white acrylic box (45 × 45 × 40 cm) illuminated at 100 lux”
Place mice in arena
Place two unfamiliar mice of same sex from different home cages in opposite corners
View evidence from paper
“Two unfamiliar mice of the same sex, originating from different home cages, were simultaneously placed in opposite corners”
Allow free interaction
Allow mice to interact freely for 10 minutes
View evidence from paper
“Each pair was allowed to interact freely for 10 min”
Record and analyze behavior
Record behavioral parameters using SMART software
View evidence from paper
“Behavioral data were analyzed using SMART software, version 3.0”