Source Paper
Lack of striatal-enriched protein tyrosine phosphatase affected the serotonin system, behavior, and brain morphology in mice
Moskaliuk V, Komleva P, Khotskin N, Arefieva A, Shevelev O et al.
Front Psychiatry • 2026
Social Interaction Test
Objective: To evaluate social behavior by measuring duration of social contacts between test mouse and juvenile intruder
This is a Social Interaction Test protocol using mice as the model organism. The procedure involves 3 procedural steps, 1 equipment items, 2 materials. Extracted from a 2026 paper published in Front Psychiatry.
Model and subjects
mice • C57BL/6-Ptpn5_KO and C57BL/6 (wild type) • male • 2-month-old • not specified
Study window
~10 minutes hands-on
Core workflow
Introduce juvenile intruder • Record social interactions • Measure social behavior
Primary readouts
- Total duration of social contacts (intruder's head and body sniffing)
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Introduce juvenile intruder
Place a 4-week-old juvenile Balb/c male into the home cage of the test mouse
View evidence from paper
“A juvenile Balb/c male (4 weeks old) was introduced to the home cage of the tested male”
Record social interactions
Monitor and register social interactions for 10 minutes using EthoStudio software
View evidence from paper
“During 10 min, social interactions were registered with the EthoStudio software”
Measure social behavior
Evaluate social behavior as the total duration of social contacts including intruder's head and body sniffing
View evidence from paper
“Social behavior was evaluated as the total duration of social contacts (intruder's head and body sniffing)”