Experiments/Tail-Pinch Stress

Source Paper

Propranolol Administration During Morphine Addiction Attenuates Reinstatement of Drug-Aversive Memories Caused by Exposure to Stressful Stimuli

Cánovas-Cabanes A, Teruel-Fernández FJ, Fernández-López L, Martínez-Laorden E, Navarro-Zaragoza J et al.

Pharmaceuticals (Basel) • 2025

PMC

Tail-Pinch Stress

behavioralmiceC57BL/6J

Objective: To assess the effect of β-adrenergic receptor blockade on tail-pinch stress-induced reinstatement of naloxone-associated conditioned place aversion

This is a Tail-Pinch Stress protocol using mice as the model organism. The procedure involves 6 procedural steps, 1 equipment items, 3 materials. Extracted from a 2025 paper published in Pharmaceuticals (Basel).

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Overview Experimental Context Protocol Vendor Comparison Materials Checklist Evidence / Verification Related Experiments

Replication Essentials

Model and subjects

mice • C57BL/6J • male • adult • 179

Study window

~1 day study window | ~15 minutes hands-on

Core workflow

Timeline initiation • Drug administration • Waiting period

Primary readouts

Time spent in naloxone-paired chamber during reinstatement test
Number of crossings between compartments

Key equipment and reagents

CPA apparatusPaper clip

Before You Start

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Protocol Steps

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Timeline initiation

Begin procedure 24 hours after extinction phase completion

View evidence from paper

“Twenty-four hours after extinction, mice were acutely administered propranolol or its vehicle”

Drug administration

Administer propranolol (10 mg/kg, i.p.) or vehicle to mice

View evidence from paper

“mice were acutely administered propranolol or its vehicle”

Waiting period

Wait 30 minutes after drug administration before stress induction

View evidence from paper

“After thirty minutes, physical stress was induced via tail-pinch”