Source Paper
Changes in gut, microbiome, and cognition after doxorubicin, cyclophosphamide, and paclitaxel chemotherapy treatment
Cronin B, Kandel S, McElroy T, Syed S, Swinton C et al.
Sci Rep • 2026
Three-Chamber Sociability Test
Objective: To assess sociability and preference for social novelty in mice
This is a Three-Chamber Sociability Test protocol using mice as the model organism. The procedure involves 5 procedural steps, 1 equipment items, 5 materials. Extracted from a 2026 paper published in Sci Rep.
Model and subjects
mice • C57/BL6J • female • twelve-week-old • not specified
Study window
~30 minutes hands-on
Core workflow
Habituation stage • Introduction of Stranger 1 • Social novelty phase
Primary readouts
- Percentage of time spent with Stranger 1 versus Stranger 2
Key equipment and reagents
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Protocol Steps
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Habituation stage
Place test mouse in center chamber and allow free exploration for 10 minutes
View evidence from paper
“the test mouse was placed in the center chamber and was allowed to freely explore for 10 min (Stage 1)”
Introduction of Stranger 1
Place Stranger 1 mouse inside a wire cage in one side chamber and allow test mouse to familiarize for 10 minutes
View evidence from paper
“a mouse of similar age, sex, and strain (Stranger 1), was placed inside a small wire cage in one of the side chambers (Stage 2). The test mouse was allowed to familiarize itself with Stranger 1 for 10 min”
Social novelty phase
Place Stranger 2 in wire cage in the other side chamber and allow test mouse to explore for 10 minutes
View evidence from paper
“a second stranger (Stranger 2) was placed in the previously empty wire cage in the other side chamber (Stage 3). The test mouse was again allowed to explore Stranger 2 for 10 min (Social Novelty phase)”
Apparatus cleaning
Clean apparatus with 20% ethanol and allow to air dry before next trial
View evidence from paper
“The apparatus was cleaned with 20% ethanol and allowed to air dry before the next trial began”
Counterbalancing
Counterbalance location of Stranger 1 versus Stranger 2
View evidence from paper
“Location of Stranger 1 versus Stranger 2 was appropriately counterbalanced”