Source Paper
Lack of striatal-enriched protein tyrosine phosphatase affected the serotonin system, behavior, and brain morphology in mice
Moskaliuk V, Komleva P, Khotskin N, Arefieva A, Shevelev O et al.
Front Psychiatry • 2026
Three-Chambered Social Approach Test
Objective: To evaluate a mouse's sociability
This is a Three-Chambered Social Approach Test protocol using mice as the model organism. The procedure involves 3 procedural steps, 2 equipment items, 1 materials. Extracted from a 2026 paper published in Front Psychiatry.
Model and subjects
mice • C57BL/6-Ptpn5_KO and C57BL/6 (wild type) • male • 2-month-old • not specified
Study window
Estimated timing pending
Core workflow
Researcher exits room • Record movement and chamber time • Clean equipment between tests
Primary readouts
- Time spent in each chamber
- Preference index calculated as time (%) spent in the chamber with the 'guest' mouse relative to total time
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Researcher exits room
The researcher leaves the testing room during the experiment
View evidence from paper
“During the time of testing, the researcher left the room”
Record movement and chamber time
Track mouse movement using 3D sensor and record time spent in each chamber
View evidence from paper
“Movement tracking was performed with Kinect 3D sensor connected to the PC through the USB-2 port”
Clean equipment between tests
Clean the device and all materials with wet and dry napkins between tests
View evidence from paper
“Between tests, the device and all used materials were cleaned with wet (H2O2) and dry napkins”