Source Paper
Olig2-specific loss-of-function Slc35a2 results in hypomyelination and spontaneous seizures
Bartel TM, Ghosh C, Bisaha K, Khoury J, Nemes-Baran A et al.
Epilepsia • 2025
Video-EEG Monitoring
behavioralmiceSlc35a2-floxed conditional knockout (cKO) mice and controls
Objective: To characterize electroencephalographic (EEG) phenotypes and analyze seizure patterns in conditional knockout mice
Protocol Steps
1
Electrode implantation
Stereotactic implantation of electroencephalographic recording electrodes prior to monitoring
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“eight control and eight cKO mice were implanted with electrodes”
2
Video-EEG recording
Record mice for up to 24 hours with simultaneous EEG and video monitoring
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“Mice were recorded for up to 24 h with simultaneous EEG and video”
3
Signal processing
Reformat digitized EEG signals to a signal channel using left-right motor cortex epidural electrodes
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“digitized EEG signals were reformatted to a signal channel (left–right motor cortex epidural electrodes)”
4
EEG analysis
Analyze EEG recordings by visual inspection for epileptiform discharges and seizure patterns
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“EEG recordings were analyzed by visual inspection for the presence of interictal epileptiform discharges”
5
Behavioral correlation
Correlate EEG seizure patterns with synchronized video of mouse behavior during ictal phase
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“then correlated with synchronized video of mouse behavior during the ictal phase”