Source Paper
Olig2-specific loss-of-function Slc35a2 results in hypomyelination and spontaneous seizures
Bartel TM, Ghosh C, Bisaha K, Khoury J, Nemes-Baran A et al.
Epilepsia • 2025
Video-EEG Monitoring
Objective: To characterize electroencephalographic (EEG) phenotypes and analyze seizure patterns in conditional knockout mice
This is a Video-EEG Monitoring protocol using mice as the model organism. The procedure involves 5 procedural steps, 1 equipment items, 1 materials. Extracted from a 2025 paper published in Epilepsia.
Model and subjects
mice • Slc35a2-floxed conditional knockout (cKO) mice and controls • male and female • postnatal day (P) 90-100 • 16
Study window
~24 hours hands-on
Core workflow
Electrode implantation • Video-EEG recording • Signal processing
Primary readouts
- interictal epileptiform discharges
- EEG seizure patterns
- synchronized behavioral changes during ictal phase
Key equipment and reagents
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
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Electrode implantation
Stereotactic implantation of electroencephalographic recording electrodes prior to monitoring
View evidence from paper
“eight control and eight cKO mice were implanted with electrodes”
Video-EEG recording
Record mice for up to 24 hours with simultaneous EEG and video monitoring
View evidence from paper
“Mice were recorded for up to 24 h with simultaneous EEG and video”
Signal processing
Reformat digitized EEG signals to a signal channel using left-right motor cortex epidural electrodes
View evidence from paper
“digitized EEG signals were reformatted to a signal channel (left–right motor cortex epidural electrodes)”
EEG analysis
Analyze EEG recordings by visual inspection for epileptiform discharges and seizure patterns
View evidence from paper
“EEG recordings were analyzed by visual inspection for the presence of interictal epileptiform discharges”
Behavioral correlation
Correlate EEG seizure patterns with synchronized video of mouse behavior during ictal phase
View evidence from paper
“then correlated with synchronized video of mouse behavior during the ictal phase”