Source Paper
Effects of Cardamom on Neuroinflammation, Learning and Memory in Mice Fed a Cafeteria Diet
AL‐Dalaeen A, Batarseh N, Atawneh S
Endocrinol Diabetes Metab • 2026
Y-Maze Test
Objective: To assess working memory in mice
This is a Y-Maze Test protocol using mice as the model organism. The procedure involves 4 procedural steps, 1 equipment items, 1 materials. Extracted from a 2026 paper published in Endocrinol Diabetes Metab.
Model and subjects
mice • C57BL/6 • male • 6-week-old • 36
Study window
~10 minutes hands-on
Core workflow
Mouse placement • Free exploration • Data recording
Primary readouts
- Sequence of arm entries
- Number of arm entries
- Spontaneous alternation behaviour
Key equipment and reagents
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Protocol Steps
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Mouse placement
Place mice in the centre of the Y-maze apparatus
View evidence from paper
“Mice were placed in the centre”
Free exploration
Allow mice to explore the maze freely for 10 minutes
View evidence from paper
“allowed to discove the box freely for 10 min”
Data recording
Record the sequence and number of arm entries during the exploration period
View evidence from paper
“The sequence and number of arm entries were recorded”
Data analysis
Calculate spontaneous alternation behaviour from the recorded data
View evidence from paper
“spontaneous alternation behaviour was calculated”