Source Paper
Behavioral effects of a chronic envy-like stress paradigm in mice using an adjacent cage model
Ueno H, Tanaka Y, Kitano E, Takahashi Y, Mori S et al.
IBRO Neurosci Rep • 2026
Y-maze test
Objective: Spatial working memory evaluation
This is a Y-maze test protocol using mice as the model organism. The procedure involves 4 procedural steps, 1 equipment items, 3 materials. Extracted from a 2026 paper published in IBRO Neurosci Rep.
Model and subjects
mice • C57BL/6 N • male • Eight-week-old • 20
Study window
~6 minutes hands-on
Core workflow
Equipment cleaning • Mouse placement • Free exploration
Primary readouts
- total distance traveled (m)
- number of arm entries
- number of alternations
- percentage of spontaneous alternations
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
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- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
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Equipment cleaning
Clean apparatus with ethanol and hypochlorous acid solution
View evidence from paper
“thoroughly cleaned with 70 % ethanol followed by a hypochlorous acid solution after each use”
Mouse placement
Place each mouse in the center of the maze
View evidence from paper
“Each mouse was placed in the center of the maze”
Free exploration
Allow mouse to freely explore for 6 minutes without prior habituation
View evidence from paper
“allowed to freely explore for 6 min. Mice were tested without prior exposure or habituation”
Data recording
Record behavioral parameters using ANY-maze software
View evidence from paper
“Behavioral parameters recorded with ANY-maze software”