Source Paper
Morin improves postoperative cognitive dysfunction by mediating the miR-138-5p/SIRT1 axis to regulate ferroptosis
Yang Y, Hu W, Wu Z, Chen M, Li H et al.
Sci Rep • 2026
Y Maze Test
behavioralmiceC57BL/6J
Objective: Spatial recognition memory test to assess cognitive function after cardiac surgery
Materials & Equipment Checklist
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Protocol Steps
1
Adaptation period
Place mice in Y maze experiment room for habituation
View evidence from paper
“On the third day after surgery, the mice were placed in the Y maze experiment room for 1 h of adaptation”
2
Testing session
Place mice at end of arm A and allow free exploration of all three arms
View evidence from paper
“On the fourth day after surgery, the mice were placed at the end of arm A and allowed to freely explore arms A, B, and C for 5 min”
3
Data recording
Record order and total number of arms entered during exploration
View evidence from paper
“The order and total number of arms entered were recorded”
4
Entry criterion
Define arm entry as all four limbs entering the arm
View evidence from paper
“The criterion for mice to enter each arm was that all four limbs entered”
5
Cleaning between trials
Clean maze with alcohol between each trial
View evidence from paper
“After each trial, the maze was treated with 75% alcohol before the next trial”