Source Paper
Morin improves postoperative cognitive dysfunction by mediating the miR-138-5p/SIRT1 axis to regulate ferroptosis
Yang Y, Hu W, Wu Z, Chen M, Li H et al.
Sci Rep • 2026
Y Maze Test
Objective: Spatial recognition memory test to assess cognitive function after cardiac surgery
This is a Y Maze Test protocol using mice as the model organism. The procedure involves 5 procedural steps, 1 equipment items, 1 materials. Extracted from a 2026 paper published in Sci Rep.
Model and subjects
mice • C57BL/6J • not specified • 8 weeks old • 6
Study window
Estimated timing pending
Core workflow
Adaptation period • Testing session • Data recording
Primary readouts
- Total number of arm entries
- Number of successful alternations
- Alternation rate calculated as: [number of successful alternations/(total number of entries into the arm-2)] × 100%
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
Confirm first
- Verify the animal model, intervention setup, and collection timepoints against the source paper.
- Check that every direct vendor link matches the exact specification your lab plans to run.
Use the page like this
- Work through the protocol steps in order and use the inline vendor chips only when you need to source or verify an item.
- Jump to Experimental Context for readouts, data shape, and analysis flow before planning downstream analysis.
Protocol Steps
Start here. The step list is optimized for running the experiment, with direct vendor links available inline when you need to source a cited item.
Adaptation period
Place mice in Y maze experiment room for habituation
View evidence from paper
“On the third day after surgery, the mice were placed in the Y maze experiment room for 1 h of adaptation”
Testing session
Place mice at end of arm A and allow free exploration of all three arms
View evidence from paper
“On the fourth day after surgery, the mice were placed at the end of arm A and allowed to freely explore arms A, B, and C for 5 min”
Data recording
Record order and total number of arms entered during exploration
View evidence from paper
“The order and total number of arms entered were recorded”
Entry criterion
Define arm entry as all four limbs entering the arm
View evidence from paper
“The criterion for mice to enter each arm was that all four limbs entered”
Cleaning between trials
Clean maze with alcohol between each trial
View evidence from paper
“After each trial, the maze was treated with 75% alcohol before the next trial”