Source Paper
Sex‐specific acceleration of Alzheimer's pathogenesis by chronic sleep‐deprivation
Wear D, Morrone CD, Simpson D, Liu F, Hussaini SA et al.
Alzheimers Dement • 2026
Y-Maze
Objective: To assess working memory in mice using spontaneous alternation behavior
This is a Y-Maze protocol using mice as the model organism. The procedure involves 7 procedural steps, 2 equipment items, 1 materials. Extracted from a 2026 paper published in Alzheimers Dement.
Model and subjects
mice • transgenic APP NL‐G‐F/NL‐G‐F knock‐in mice • male and female • 6 months • 32
Study window
~35 minutes hands-on
Core workflow
Timing • Room habituation • Mouse placement
Primary readouts
- Spontaneous alternation percentage calculated as: No. of Correct Alternations/(No. of Arm Entries − 2) × 100%
Key equipment and reagents
Use this page as an execution guide, then fall back to the source paper whenever you need exact exclusions, dosing details, or assay-specific caveats.
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- Verify the animal model, intervention setup, and collection timepoints against the source paper.
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Protocol Steps
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Timing
Conduct Y-maze test nine days after sleep disruption period
View evidence from paper
“Nine days after SD, mice were subjected to a Y‐maze test”
Room habituation
Allow mice to habituate to the testing room for minimum 30 minutes
View evidence from paper
“Mice were habituated to the testing room for a minimum of 30 min prior to experimentation”
Mouse placement
Place mice in one arm facing the arm's end
View evidence from paper
“Mice were placed in one arm facing the arm's end”
Exploration period
Allow mice to explore the maze for 5 minutes
View evidence from paper
“and allowed to explore the maze for 5 min”
Recording
Record trials and manually score arm entries from videos
View evidence from paper
“each arm entry was recorded manually from the obtained videos”
Arm entry criteria
Count arm entry when entire mouse body enters the arm
View evidence from paper
“An arm entry was indicated when the entire mouse had entered the arm”
Alternation scoring
Score correct alternation as entering each of three arms in sequence without returning to previously investigated arm
View evidence from paper
“A correct alternation was the mouse entering each of the three arms in sequence without returning to the previously investigated arm”