Source Paper
Sex‐specific acceleration of Alzheimer's pathogenesis by chronic sleep‐deprivation
Wear D, Morrone CD, Simpson D, Liu F, Hussaini SA et al.
Alzheimers Dement • 2026
Y-Maze
Objective: To assess working memory in mice using spontaneous alternation behavior
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Protocol Steps
Timing
Conduct Y-maze test nine days after sleep disruption period
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“Nine days after SD, mice were subjected to a Y‐maze test”
Room habituation
Allow mice to habituate to the testing room for minimum 30 minutes
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“Mice were habituated to the testing room for a minimum of 30 min prior to experimentation”
Mouse placement
Place mice in one arm facing the arm's end
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“Mice were placed in one arm facing the arm's end”
Exploration period
Allow mice to explore the maze for 5 minutes
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“and allowed to explore the maze for 5 min”
Recording
Record trials and manually score arm entries from videos
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“each arm entry was recorded manually from the obtained videos”
Arm entry criteria
Count arm entry when entire mouse body enters the arm
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“An arm entry was indicated when the entire mouse had entered the arm”
Alternation scoring
Score correct alternation as entering each of three arms in sequence without returning to previously investigated arm
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“A correct alternation was the mouse entering each of the three arms in sequence without returning to the previously investigated arm”